Small Animal in Vivo Imaging System

Product name	Label-free multimodal in vivo imaging of small animals
Serial version	Standard Edition		Tunable wavelength version
Model	GAni Standard Edition	GAni-Plus Upgrade	GAni-OPO	GAni-OPO Ultimate
Imaging modality	Photoacoustic & Optical & Ultrasound Imaging	Dual-wavelength photoacoustic & ultrasound imaging	Photoacoustic & Ultrasound Imaging	Multi-wavelength photoacoustic & ultrasound imaging
Application direction	Brain, organs, tumors, blood vessels	Brain, organs, tumors, skin, blood vessels, pigments	Brain, organs, tumors, skin, molecular probes, blood vessels, pigments, NIR-I materials	Brain, organs, tumors, skin, molecular probes, blood vessels, pigments, lipids, NIR-I materials, NIR-II materials
Wavelength range	532nm	532nm& 1064nm	532nm OPO(770-840nm) 1064nm	532nm OPO(680-1190nm & 1150-2400nm) 1064nm
Imaging range	3x3 mm, 1min	3x3 mm, 1min	3x3 mm, 1min	3x3 mm, 1min
Imaging time	20x20 mm, 20min	20x20 mm, 20min	20x20 mm, 20min	20x20 mm, 20min
Lateral resolution	3μm	3μm	3μm	3μm
Axial resolution	75μm	75μm	75μm	75μm
Measurement depth	3mm	6 mm	6 mm	6 mm

 

 

GCell Multimodal small animal in vivo imaging system is a small animal in vivo imaging system that uses a variety of imaging technologies for comprehensive imaging, which can simultaneously detect and analyze the physiology, pathology, efficacy and other information of small animals. This technology can improve the accuracy and sensitivity of imaging, and provide more comprehensive and in-depth data support for biomedical research and drug development.

 

 

GCell in vivo imaging system becoming increasingly popular due to their numerous advantages. Here are some of the most important benefits of this product:
1. Optical/photoacoustic/ ultrasound three-modal imaging
A three-modal in vivo small animal imaging system that integrates optical microscopy, photoacoustic imaging of endogenous light-absorbing substances such as pigments and blood vessels, and ultrasound imaging of acoustic impedance differences.

2. Micron-level resolution, millimeter-level imaging depth
Micron, high-resolution imaging of tissue structures within 3 mm can still be performed without the need for contrast media, and the position of the focus can be adjusted according to the real-time display of the software.

3. Three-dimensional image information is analyzed layer by layer
Through the real-time 2D tomographic data display overlay, the 3D structural images of the local tissue can be further obtained, and the 2D and 3D images can be further analyzed by using data processing software.

4. Non-invasive, label-free imaging
Only a small amount of water (couplant) is applied to the imaging site to match the signal, and non-invasive imaging of the test site can be achieved without the injection of contrast agent.

5. Heating-anesthesia-integrated small animal fixation table
Integrated heating-anesthesia device specifically designed for better protection of model animals.

6. Imaging systems with customized light sources
According to the different needs of customers, customize the corresponding single-wavelength, multi-wavelength, tunable wavelength light source imaging system.

 

 

GCell in vivo imaging system are widely used in below area
1. Monitoring of tumor growth process
The monitoring of the growth of tumor trophic blood vessels in the ears of mice, the monitoring of the growth of tumor trophic blood vessels, and the relationship between the curvature, density and depth of tumor trophic blood vessels and the tumor growth time were verified.

 

References
[1]. F. Yang, et al..J. Biophotonics, e202000022.2020.DOI:10.1002/-jbio.20000022
[2]. Z. Wang, Nanophotonics,10(12), 3359-3368, 2021.DOI:10.1515/nanoph-2021-0198.

 

2. Monitoring of the treatment process of tumors
The monitoring of the ablation of the nourishing vessels during the photodynamic (PDT) treatment of back tumors in mice was realized, and the relationship between the curvature, density and depth of the tumor trophic vessels and the duration of PDT treatment was revealed.

References
F. Yang, et al.,J. Biophotonics, e202000022.2020, DOI:10.1002/-jbio.20000022.

 

3. Functional imaging of the brain in small animals
The dynamic monitoring of "ischemia-reperfusion" of the vascular network deep in the mouse brain was realized, and the broad application prospect of this instrument in the basic research of cerebrovascular diseases was demonstrated.

 

References
F.Yang. et al.. J. Biophotonics, e202000022.2020.DOI:10.1002/- jbio.20000022

 

4. Assess the extent of blood supply to the lesions
The evaluation of the degree of blood supply to the back of mice and the total retreat of mice was realized, which broke through the bottleneck of imaging technology to evaluate the degree of blood supply to damaged tissues and improved the possibility of rapid surgical intervention.

References
D.Zhang.et al., Quant Imaging Med Surg, 11(10).4365-4374.2021.DOI:10.21037/qims-21-135.

 

5. Imaging of iris and sclera in live animals
It can realize the imaging of the iris and scleral vascular network of the eyes of live small animals (such as mice) and large animals (such as rabbits).

 

6. Nanoprobes and molecular imaging studies
Tumor-specific photoacoustic imaging at special wavelengths (custom version)
The photoacoustic multi-modal small animal imager can be customized, and the specific nanoprobe can be used to improve the amplitude of the photoacoustic imaging signal of the tumor area for special wavelengths, so as to achieve large-depth and high-sensitivity tumor-specific photoacoustic imaging.

References
[1]. D.Cui, et al.. Nano Letters, 21(16).6914-6922.2021, DOI:10.1021/acs. nanolett.1c02078[2]. J.Zheng. et al., J. Am. Chem. Soe,141(49),19226-19230.2019.DOI: 10.1021/jacs.9b10353.

 

7. Breast tumor specimen marker imaging
T.Wong.et_x0001_al.. _x0001_Sci.Adv.,3_x0001_(5)._x0001_e1602168.2017.D01:_x0001_10.1126/sciadv.1602168.
Labeled imaging of liver micrometastases in early-stage neoma
Q.Yu,et_x0001_al.,J_x0001_Nucl_x0001_Med. 61(7),10791085,2020.00I:_x0001_10.2967/inumed.119.23315

 

8. Ambulatory monitoring of structural and functional changes in the early stages of abscient stroke
J.Lv.et_x0001_al.,_x0001_Theranostics,10(2).816-828.2020.DOI:10.7150/thno.38554.
Multimodal imaging observations of the living eye before and after suture injury
J.Park.B.Park.et_x0001_al.,_x0001_PNAS.118(11)._x0001_e1920879118.2021,_x0001_DO1:10.1073/pnas.1920879118.
Imaging of the retina in live animals, choroid, iris, sclera
C.Tian,_x0001_et_x0001_al.,_x0001_0ptics_x0001_Express,25(14)._x0001_15947-15955,2017.DOI:10.1364/0E.25.015947.
Z.Hosseinace,_x0001_et_x0001_al.,_x0001_Optics_x0001_Letters,45(22).6254-6257,2020.DOI:10.1364/0L.410171.
Labeled imaging of cells in the liver
D. Deng.et_x0001_al.,Nanophotonics,2021,DOI:/10.1515/nanoph-2021-0281.

 

9. Quantitative assessment of pigment distribution
The photoacoustic multimodal imaging system can quantitatively assess skin pigmentation and assist in clinical diagnosis

References
H.Ma. et al., Appl, Phys, Lett.. 113,083704,2018.DOI:10.1063/1.5041769.

 

10. Microvascular quantitative assessment
The photoacoustic multimodal imaging system can quantitatively monitor the effect of bright erythema before and after treatment, and give the most intuitive feedback on pathological parameters

Reference

H. Ma. et al.. Bio. Exp.12(10).6300-6316.2021.DOI:10.1364/B0E.439625.
Two-dimensional assessment Three-dimensional quantification Pre- and post-treatment evaluation

 

 

Q1. For nanomaterials, how to obtain photoacoustic imaging results with a high signal-to-noise ratio?
1. Select the appropriate wavelength of laser to match the absorption peak of the nanomaterial. This enhances the photoacoustic signal;
2. Select high-frequency probes to improve the detection ability of weak acoustic signals generated by nanomaterials;
3. Ensure that the nanomaterials are evenly distributed in the sample, avoiding aggregation and clustering, to obtain a uniform photoacoustic signal.
4. Consider using contrast agents to enhance the photoacoustic signature of nanomaterials, such as labeling the surface of nanoparticles with substances that absorb strongly.

Q2. Will the resolution decrease as the depth increases?
As the depth increases, the laser excitation decreases, and the signal decreases, so the resolution decreases; However, in the field of photoacoustic microscopy, our photoacoustic multimodal imaging has the highest resolution at large depths.

Q3. Does photoacoustic microscopy need to be laparotomy to image the internal organs of small animals, and does craniotomy be required to image the brain?
1. Imaging the distribution of fine blood vessels or materials at different levels of liver, kidney, stomach, intestines, uterus, testicles, etc., requires laparotomy.
2. For brain function, observe the distribution of fine blood vessels or materials at different levels of the brain, without craniotomy.
3. For the heart and lungs, when imaging in vivo, it is necessary to overcome the imaging blur caused by physiological movements such as heart beating and breathing; As a result, in ex vivo conditions, motion artifacts are reduced and image quality is higher.

Q4. Can ex vivo organs be imaged?
Newly removed organs can be directly scanned for imaging; If the organ has been out of body for too long and there is too much blood loss, the morphological structure of the blood vessel can be imaged by perfusion of contrast medium, and the absorption wavelength of the contrast medium must be in the wavelength range of the laser.